CDK4/cyclin D1 Kinase Assay Service

Activity-based CDK4/cyclin D1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK4/Cyclin D1
Enzyme
Human CDK4/Cyclin D1
HGNC
CDK4/CCND1
Synonym
CMM3, PSK-J3
Substrates
RB protein, 3 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
CDK4: P11802 (http://www.uniprot.org/uniprot/P11802)Cyclin D1: P24385 (http://www.uniprot.org/uniprot/P24385)
Screening Location
Malvern, USA
Reference compound IC50 for CDK4/Cyclin D1