CDK4/cyclin D2 Kinase Assay Service

Activity-based CDK4/cyclin D2 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK4/Cyclin D2

Enzyme

Human CDK4/Cyclin D2

HGNC

CDK4/CCND2

Synonym

CDK4: Cell division protein kinase 4, Cyclin-dependent kinase 4, PSK-J3
Cyclin D2: G1/S-specific cyclin-D2, CCND2

Substrates

RB protein, 3 µM
ATP, 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

CDK4: P11802 (http://www.uniprot.org/uniprot/P11802)
Cyclin D2: P30279 (http://www.uniprot.org/uniprot/P30279)

Screening Location

Malvern, USA

Reference compound IC50 for CDK4/Cyclin D2