CDK6/cyclin D1 Kinase Assay Service

Activity-based CDK6/cyclin D1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK6/Cyclin D1
Enzyme
Human CDK6/Cyclin D1
HGNC
CDK6/CCND1
Synonym
PLSTIRE
Substrates
RB protein, 3 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine2.2
Ro 31-82207.83
GW 507413,800
H-89>100,000
Rottlerin20,500
Scientific Information
CDK6: Q00534 (http://www.uniprot.org/uniprot/Q00534)Cyclin D1: P24385 (http://www.uniprot.org/uniprot/P24385)
Screening Location
Malvern, USA
Reference compound IC50 for CDK6/cyclin D1
Reference compound IC50 for CDK6/cyclin D1