CDK6/cyclin D2 Kinase Assay Service

Activity-based CDK6/cyclin D2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

CDK6/cyclin D2

Enzyme

Human CDK6/Cyclin D2

HGNC

CDK6-CCND2

Synonym

CDK6: Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE
Cyclin D2: G1/S-specific cyclin-D2, CCND2

Substrates

RB protein, 3 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 47.4
Ro 31-8220 21.2
GW 5074 6,500
H-89 >100,000
Rottlerin >100,000
Scientific Information

CDK6: Q00534 (http://www.uniprot.org/uniprot/Q00534)
Cyclin D2: P30279 (http://www.uniprot.org/uniprot/P30279)

Screening Location

Malvern, USA

Reference compound IC50 for CDK6/cyclin D2

Reference compound IC50 for CDK6/cyclin D2