CDK7/cyclin H Kinase Assay Service

Activity-based CDK7/cyclin H kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK7/Cyclin H/MNAT1
Enzyme
Human CDK7/Cyclin H/MNAT1
HGNC
CDK7/CCNDH
Synonym
CAK1, CDKN7, STK1, p39MO15
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine115
Ro 31-8220160
GW 5074>100,000
H-8911,300
Rottlerin>100,000
Scientific Information
CDK7: P50613 (http://www.uniprot.org/uniprot/P50613)Cyclin H: P51946 (http://www.uniprot.org/uniprot/P51946)MNAT1: P51948 (http://www.uniprot.org/uniprot/P51948)
Screening Location
Malvern, USA
Reference compound IC50 for CDK7/cyclin H
Reference compound IC50 for CDK7/cyclin H