CDK7/cyclin H Kinase Assay Service

Activity-based CDK7/cyclin H kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CDK7/Cyclin H/MNAT1

Enzyme

Human CDK7/Cyclin H/MNAT1

HGNC

CDK7/CCNDH

Synonym

CAK1, CDKN7, STK1, p39MO15

Substrates

MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
GW 5074	>100,000
H-89	11,300
Ro 31-8220	160
Rottlerin	>100,000
Staurosporine	115

Scientific Information

CDK7: P50613 (http://www.uniprot.org/uniprot/P50613)Cyclin H: P51946 (http://www.uniprot.org/uniprot/P51946)MNAT1: P51948 (http://www.uniprot.org/uniprot/P51948)

Screening Location

Malvern, USA

Reference compound IC50 for CDK7/cyclin H