CDK9/cyclin K Kinase Assay Service

Activity-based CDK9/cyclin K kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CDK9/Cyclin K

Enzyme
Human CDK9/Cyclin K

HGNC
CDK9/CCNK

Synonym
C-2k, CDC2L4, PITALRE, TAK, CDC2-related kinase

Substrates
Peptide substrate, [KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 7.2
Ro 31-8220 <1.0
GW 5074 5,600
H-89 6,300
Rottlerin >100,000
Scientific Information
CDK9: P50750 (http://www.uniprot.org/uniprot/P50750)Cyclin K: O75909 (http://www.uniprot.org/uniprot/O75909)

Screening Location
Malvern, USA

Reference compound IC50 for CDK9/cyclin K
Reference compound IC50 for CDK9/cyclin K