Activity-based CDK9/cyclin K kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK9/Cyclin K
Enzyme
Human CDK9/Cyclin K
HGNC
CDK9/CCNK
Synonym
C-2k, CDC2L4, PITALRE, TAK, CDC2-related kinase
Substrates
Peptide substrate, [KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds | IC50 (nM) |
---|---|
Staurosporine | 7.2 |
Ro 31-8220 | <1.0 |
GW 5074 | 5,600 |
H-89 | 6,300 |
Rottlerin | >100,000 |
Scientific Information
Screening Location
Malvern, USA
Reference compound IC50 for CDK9/cyclin K
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