Activity-based CDK9/cyclin T1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CDK9/Cyclin T1
Enzyme
Human CDK9/Cyclin T1
HGNC
CDK9/CCNT1
Synonym
C-2k, CDC2L4, PITALRE, TAK, CDC2-related kinase
Substrates
Peptide substrate, [KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds | IC50 (nM) |
---|---|
Staurosporine | 14 |
Ro 31-8220 | 10 |
GW 5074 | 8,500 |
H-89 | 12,000 |
Scientific Information
P50750 (http://www.uniprot.org/uniprot/P50750)
Screening Location
Malvern, USA
Reference compound IC50 for CDK9/cyclinT1
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