CDK9/cyclin T2 Kinase Assay Service

Activity-based CDK9/cyclin T2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CDK9/Cyclin T2

Enzyme
Human CDK9/Cyclin T2

HGNC
CDK9/CCNT2

Synonym
C-2k, CDC2L4, PITALRE, TAK, CDC2-related kinase

Substrates
Peptide substrate, [KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 6.4
Ro 31-8220 <1.0
GW 5074 5,000
H-89 5,400
Rottlerin 17,000
Scientific Information
CDK9: P50750 (http://www.uniprot.org/uniprot/P50750)Cyclin T2: O60583 (http://www.uniprot.org/uniprot/O60583)

Screening Location
Malvern, USA

Reference compound IC50 for CDK9/cyclin T2
Reference compound IC50 for CDK9/cyclin T2

PDF