CDK9+Cyclin T2 NanoBRET Kinase Assay

CDK9+Cyclin T2 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview


CDK9+Cyclin T2

HGNC Symbol

CDK9/CCNT2 complex



Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-CDK9 Fusion Vector and Cyclin T2 Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-10 and then treated with reference compound Dinaciclib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.


Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
Dinaciclib 5.877e-009

Screening Location

Malvern, USA

Reference compound IC50 for CDK9+Cyclin T2