CHK1 Kinase Assay Service

Activity-based CHK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CHK1
Enzyme
Human CHK1
HGNC
CHEK1
Synonym
CHEK1
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine0.3
Ro 31-822018.7
GW 50745,400
H-891,200
Rottlerin>100,000
Scientific Information
O14757 (http://www.uniprot.org/uniprot/O14757)
Screening Location
Malvern, USA
Reference compound IC50 for CHK1
Reference compound IC50 for CHK1