Activity-based CIT 1-450 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CIT 1-450
Enzyme
Human CIT 1-450, wt
HGNC
CIT
Manning ID
CRIK
Synonym
STK2, STK21, KIAA0949, DRIK, CTRO, STK21/CIT, CIT
UniProt
O14578 (CTRO_HUMAN)
Kinase Type
Protein kinase
Kinase Group
AGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Histon H2B, ATP
Reaction
Histon H2B + 33P-ATP = 33P-Histon H2B + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1200 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CIT 1-450 |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – CIT 1-450 |
| Product Number | 0370-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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