Activity-based CK1a1 (E42C) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CK1a1 (E42C)
Enzyme
Human CK1a1 (E42C)
HGNC
CK1a1 (E42C)
Synonym
Casein kinase I isoform alpha, CKI-alpha, CK1, KC1A, CSNK1A1
Scientific Information
P48729 (http://www.uniprot.org/uniprot/P48729)
Assay Properties
Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2,500 |
| Ro 31-8220 | 12,000 |
| GW5074 | 3,300 |
| H-89 | 17,000 |
| Rottlerin | 7,100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for CK1a1 (E42C)
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