CK1a1 (I35C) Kinase Assay Service

Activity-based CK1a1 (I35C) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

CK1a1 (I35C)

Enzyme

Human CK1a1 (I35C)

HGNC

CK1a1 (I35C)

Synonym

Casein kinase I isoform alpha, CKI-alpha, CK1, KC1A, CSNK1A1

Scientific Information

P48729 (http://www.uniprot.org/uniprot/P48729)

Assay Properties

Substrates

Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 8,000
Ro 31-8220 19,000
GW5074 6,700
H-89 32,000
Rottlerin 80,000

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for CK1a1 (I35C)

Reference compound IC50 for CK1a1 (I35C)