CK1a1 Kinase Assay Service

Activity-based CK1a1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CK1a1
Enzyme
Human CK1a1
HGNC
CSNK1A1
Synonym
CK1, HLCDGP1, PRO2975, CK1-alpha1
Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine7,800
GW 507430,500
Scientific Information
P48729 (http://www.uniprot.org/uniprot/P48729)
Screening Location
Malvern, USA
Reference compound IC50 for CK1a1
Reference compound IC50 for CK1a1