Activity-based CK1a1L kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CK1a1L
Enzyme
Human CK1a1L
HGNC
CSNK1A1L
Synonym
CK1-alpha1 like
Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3,600 |
| Ro 31-8220 | 35,000 |
| GW5074 | 8,800 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q8N752 (http://www.uniprot.org/uniprot/Q8N752)
Screening Location
Malvern, USA
Reference compound IC50 for CK1a1L
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