CK1a1L Kinase Assay Service

Activity-based CK1a1L kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CK1a1L
Enzyme
Human CK1a1L
HGNC
CSNK1A1L
Synonym
CK1-alpha1 like
Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine3,600
Ro 31-822035,000
GW50748,800
H-89>100,000
Rottlerin>100,000
Scientific Information
Q8N752 (http://www.uniprot.org/uniprot/Q8N752)
Screening Location
Malvern, USA
Reference compound IC50 for CK1a1L
Reference compound IC50 for CK1a1L