CK1d Kinase Assay Service

Activity-based CK1d kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CK1d

Enzyme
Human CK1d

HGNC
CSNK1D

Synonym
HCKID, CK1-delta, CK1d

Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 12,000
Ro 31-8220 20,000
GW 5074 13,500
H-89 17,600
D4476 167
Scientific Information
P48730 (http://www.uniprot.org/uniprot/P487230)

Screening Location
Malvern, USA

Reference compound IC50 for CK1d
Reference compound IC50 for CK1d

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