CK1d Kinase Assay Service

Activity-based CK1d kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CK1d
Enzyme
Human CK1d
HGNC
CSNK1D
Synonym
HCKID, CK1-delta, CK1d
Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine12,000
Ro 31-822020,000
GW 507413,500
H-8917,600
D4476167
Scientific Information
P48730 (http://www.uniprot.org/uniprot/P487230)
Screening Location
Malvern, USA
Reference compound IC50 for CK1d
Reference compound IC50 for CK1d