CK1epsilon Kinase Assay Service

Activity-based CK1epsilon kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CK1epsilon

Enzyme
Human CK1epsilon

HGNC
CSNK1E

Synonym
CKIe, HCKIE, MGC10398, CK1-epsilon

Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 3,600
Ro 31-8220 10,100
GW 5074 2,600
H-89 66,800
D4476 350
Scientific Information
P49674 (http://www.uniprot.org/uniprot/P49674)

Screening Location
Malvern, USA

Reference compound IC50 for CK1epsilon
Reference compound IC50 for CK1epsilon