CK1epsilon Kinase Assay Service

Activity-based CK1epsilon kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CK1epsilon
Enzyme
Human CK1epsilon
HGNC
CSNK1E
Synonym
CKIe, HCKIE, MGC10398, CK1-epsilon
Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine3,600
Ro 31-822010,100
GW 50742,600
H-8966,800
D4476350
Scientific Information
P49674 (http://www.uniprot.org/uniprot/P49674)
Screening Location
Malvern, USA
Reference compound IC50 for CK1epsilon
Reference compound IC50 for CK1epsilon