CK2a2 Kinase Assay Service

Activity-based CK2a2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CK2a2

Enzyme
Human CK2a2

HGNC
CSNK2A2

Synonym
CK2-alpha2

Substrates
Peptide substrate, [RRRDDDSDDD], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 600
Ro 31-8220 3,500
GW 5074 180
H-89 >100,000
Rottlerin >100,000
Scientific Information
P19784 (http://www.uniprot.org/uniprot/P19784)

Screening Location
Malvern, USA

Reference compound IC50 for CK2a2
Reference compound IC50 for CK2a2

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