CLK2 Kinase Assay Service

Activity-based CLK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CLK2

Enzyme
Human CLK2

HGNC
CLK2

Synonym
MGC61500, hCLK2

Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 8.7
Ro 31-8220 8.8
GW 5074 8,200
H-89 31,000
Scientific Information
P49760 (http://www.uniprot.org/uniprot/P49760)

Screening Location
Malvern, USA

Reference compound IC50 for CLK2
Reference compound IC50 for CLK2