Activity-based CLK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CLK3
Enzyme
Human CLK3
HGNC
CLK3
Synonym
PHCLK3, PHCLK3/152, CDc-like kinase 3
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3,900 |
| Ro 31-8220 | 3,900 |
| GW 5074 | 46,200 |
Scientific Information
P49761 (http://www.uniprot.org/uniprot/P49761)
Screening Location
Malvern, USA
Reference compound IC50 for CLK3
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