CLK4 Kinase Assay Service

Activity-based CLK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CLK4

Enzyme
Human CLK4

HGNC
CLK4

Synonym
DKFZp686A20267, CDc-like kinase 4

Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 10.5
Ro 31-8220 16
GW 5074 1,800
H-89 7,400
Rottlerin 34,000
Scientific Information
Q9HAZ1 (http://www.uniprot.org/uniprot/Q9HAZ1)

Screening Location
Malvern, USA

Reference compound IC50 for CLK4
Reference compound IC50 for CLK4