COMT Methyltransferase Assay Service

Activity-based COMT Methyltransferase Assay for compound screening and profiling via FlashPlate methyltranferase assay.

Target Overview
CAT#

COMT

Enzyme

Human COMT

Synonym

Catechol O-methyltransferase

Substrates

COMT-S01, 0.5 µMSAM, 1 µM

Assay Properties
Reaction

S-adenosyl-L-[methyl-³H]methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol [methyl-³H]

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Tolcapone 1.5
Entacapone 0.6
Ro 41-0960 0.6
Scientific Information

P21964 (http://www.uniprot.org/uniprot/P21964)

Screening Location

Malvern, USA

Reference compound IC50 for COMT

Reference compound IC50 for COMT