COT Kinase Activity Assay Service

Activity-based COT kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview
RBE Target ID

COT

Enzyme

Human COT, wt

HGNC

MAP3K8

Manning ID

COT

Synonym

EST, ESTF, TPL2, Tpl-2, c-COT, MEKK8, MAP3K8, COT1, COT, COT1/MAP3K8

UniProt

P41279 (M3K8_HUMAN)

Kinase Type

Protein kinase

Kinase Group

STE

Assay Properties
Assay Format

33PanQinaseTM

Substrates

RBER-CHKtide, ATP

Reaction

RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
MCE 55
Sorafenib 10000
Sunitinib 100000
Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability
Screening Opportunities
FreeChoice COT
355 wt Profiler Yes
355 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – COT
(Activity verified by 33PanQinaseTM-Assay)
Product Number 0180-0000-1
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