CREBBP Bromodomain Assay Service (ThermoShift)

CREBBP-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-CREBBP-H

Protein

Human CREBBP bromodomain, His-tagged

Synonyms

CREB-binding protein, CBP

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (6.0 M)

Concentration of compound in assay

25 M

Apo form Tm (°C)

42,5

Reference Compound ΔTms:

SGC-CBP30 - 9.5 (Red)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, RVX-208, GSK2801 and PFI3)

Scientific Information

Q92793 (http://www.uniprot.org/uniprot/Q92793)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak