CTK/MATK Kinase Assay Service

Activity-based CTK/MATK kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CTK

Enzyme

Human CTK

HGNC

MATK

Synonym

CHK, CTK, DKFZp434N1212, HHYLTK, HYL, Lsk, MGC1708, MGC2101

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	770

Scientific Information

P42679 (http://www.uniprot.org/uniprot/P42679)

Screening Location

Malvern, USA

Reference compound IC50 for CTK/MATK/HYL