CYP19A for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
CYP19A
Enzyme
Human CYP19A (recombinant protein)
Synonym
Aromatase, Cytochrome P450 19A1, Estrogen synthase, ARO1, CP19A
Substrate
Fluorescent substrate
Assay Properties
Reaction
Testosterone + 3 [reduced NADPH-hemoprotein reductase] + 3 O₂ = 17-betaestradiol + formate + 4 H₂O + 3 [oxidized NADPH-hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
P11511 (http://www.uniprot.org/uniprot/P11511)
Screening Location
Malvern, USA
Further information
More information can be found on our website Cytochrome P450 Assay Services.
Reference compound IC50 for CYP19A
