CYP1A2 for compound screening and profiling assay using a luminescence-based method to measure cytochrome P450 enzyme activity.
Target Overview
CAT#
CYP1A2
Enzyme
Human CYP1A2 + P450 Reductase microsomes
Synonym
CYP1A2; cytochrome P450 family 1 subfamily A member 2; aryl hydrocarbon hydroxylase; cholesterol 25-hydroxylase
Scientific Information
PO5177 (https://www.uniprot.org/uniprotkb/P05177/entry)
Assay Properties
Substrate
P450-Glo™ proluciferin Substrate
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + oxidized [NADPH—hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Furafylline | 1620 |
| Famotidine | >10000 |
| Ketoconazole | >10000 |
| Imipramine | >10000 |
| Tranylcypromine | >10000 |
| Cimetidine | >10000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP1A2
