CYP1A2 for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
Cyp1A2
Enzyme
Cyp1A2 + NADPH-P450 Reductase (microsomes from insect cells that were infected with baculovirus containing the human Cyp1A2 cDNA)
Synonym
cytochrome P450, family 1, subfamily A, polypeptide 2, CYP 1A2
Substrate
Fluorescent substrate
Assay Properties
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (µM) |
|---|---|
| Ketoconazole | 2.05 |
| Proadifen (SKF525A) | 3.15 |
Scientific Information
NP_000752 (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=73915100)
Screening Location
Malvern, USA
Further Information
More information can be found on our website Cytochrome P450 Assay Services.
Reference compound IC50 for Cyp1A2
