CYP1A2 Inhibition Assay Service

CYP1A2 for compound screening and profiling via fluorescence-based quantification of enzyme activity.

Target Overview
CAT#

Cyp1A2

Enzyme

Cyp1A2 + NADPH-P450 Reductase (microsomes from insect cells that were infected with baculovirus containing the human Cyp1A2 cDNA)

Synonym

cytochrome P450, family 1, subfamily A, polypeptide 2, CYP 1A2

Substrate

Fluorescent substrate

Assay Properties
Reaction

RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺

Readout

Enzymatic activity

Reference Compound IC50s
Compound IC50 (µM)
Ketoconazole 2.05
Proadifen (SKF525A) 3.15
Scientific Information

NP_000752 (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=73915100)

Screening Location

Malvern, USA

Further Information

More information can be found on our website Cytochrome P450 Assay Services.

Reference compound IC50 for Cyp1A2

Reference compound IC50 for Cyp1A2