CYP2A6 for compound screening and profiling via fluorescence-based cytochrome P450 inhibition assay quantification of enzyme activity.
Target Overview
CAT#
CYP2A6
Enzyme
Human CYP2A6 + NADPH-P450 Reductase (microsomes from insect cells infected with baculovirus containing the human CYP2A6 cDNA and rabbit Cytochrome P450 cDNA)
Synonym
Cytochrome P450, family 2, subfamily A, polypeptide 6
Substrate
Fluorescence Substrate Vivid® CC
Assay Properties
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Ketoconazole | > 10,000 |
| Terfenadine | > 10,000 |
| Tranylcypromine | 21.1 |
Scientific Information
P11509 (https://www.uniprot.org/uniprotkb/P11509/entry)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2A6
