CYP2A6 for compound screening and and profiling assay using luminescence-based method to measure cytochrome P450 enzyme activity.
Target Overview
CAT#
CYP2A6
Enzyme
Human CYP2A6 + P450 Reductase microsomes
Synonym
CYP2A6; cytochrome P450 family 2, subfamily A, polypeptide 6; 1,4-cineole 2-exo-monooxygenase; coumarin 7-hydroxylase
Scientific Information
P11509 (https://www.uniprot.org/uniprotkb/P11509/entry)
Assay Properties
Substrate
P450-Glo™ proluciferin Substrate
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + oxidized [NADPH—hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Ketoconazole | 2940 |
| Miconazole | 1700 |
| Tranylcypromine | 9260 |
| Verapamil | >10000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2A6
