CYP2C19 Inhibition Assay Service

CYP2C19 for compound screening and profiling via fluorescence-based cytochrome P450 inhibition assay quantification of enzyme activity.

Target Overview
CAT#

CYP2C19

Enzyme

CYP2C19 + NADPH-P450 Reductase (microsomes from insect cells infected with baculovirus containing the human CYP2C19 cDNA and rabbit Cytochrome P450 cDNA)

Synonym

Cytochrome P450, family 2, subfamily C, polypeptide 19

Substrate

Fluorescence Substrate Vivid® EOMCC

Assay Properties
Reaction

RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺

Readout

Enzymatic activity

Reference Compound IC50s
Scientific Information

P33261 (https://www.uniprot.org/uniprotkb/P33261/entry)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website CYP450 Inhibition Assay Services.

Reference compound IC50 for CYP2C19