CYP2C8 for compound screening and profiling via fluorescence-based cytochrome P450 inhibition assay quantification of enzyme activity.
Target Overview
CAT#
CYP2C8
Enzyme
CYP2C8 + NADPH-P450 Reductase (microsomes from insect cells infected with baculovirus containing the human CYP2C8 cDNA and rabbit Cytochrome P450 cDNA)
Synonym
Cytochrome P450, family 2, subfamily C, polypeptide 8
Substrate
Fluorescence Substrate Vivid® DBOMF
Assay Properties
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (µM) |
|---|---|
| Ketoconazole | >20 |
| Miconazole | 10.4 |
| Montelukast | 0.54 |
Scientific Information
P10632 (https://www.uniprot.org/uniprotkb/P10632/entry)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2C8
