CYP2C8 for compound screening and profiling assay using luminescence-based method to measure cytochrome P450 enzyme activity.
Target Overview
CAT#
CYP2C8
Enzyme
Human CYP2C8 + P450 Reductase microsomes
Synonym
CYP2C8; cytochrome P450 family 2, subfamily C, polypeptide 8; S-mephenytoin 4-hydroxylase
Scientific Information
P10632 (https://www.uniprot.org/uniprotkb/P10632/entry)
Assay Properties
Substrate
P450-Glo™ proluciferin Substrate
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + oxidized [NADPH—hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Ketoconazole | 1300 |
| Montelukast | 55.3 |
| Tranylcypromine | >10000 |
| Cimetidine | >10000 |
| Imipramine | >10000 |
| Verapamil | >10000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2C8
