CYP2D6 Inhibition Assay Service

CYP2D6 for compound screening and profiling via fluorescence-based quantification of enzyme activity.

Target Overview
CAT#

Cyp2D6

Enzyme

Cyp2D6 + NADPH-P450 Reductase (microsomes from insect cells that were infected with baculovirus containing the human Cyp2D6 cDNA and rabbit Cytochrome P450 cDNA)

Synonym

Cytochrome P450, family 2, subfamily D, polypeptide 6

Substrate

Fluorescent substrate

Assay Properties
Reaction

RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺

Readout

Enzymatic activity

Reference Compound IC50s
Compound IC50 (µM)
Ketoconazole 1.76
Proadifen (SKF-525A) 0.03
Aminobenzotriazole 4.00
Scientific Information

NP_000097(http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=40805836)

Screening Location

Malvern, USA

Further Information

More information can be found on our website Cytochrome P450 Assay Services.

Reference compound IC50 for Cyp2D6

Reference compound IC50 for Cyp2D6