CYP2D6 for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
Cyp2D6
Enzyme
Cyp2D6 + NADPH-P450 Reductase (microsomes from insect cells that were infected with baculovirus containing the human Cyp2D6 cDNA and rabbit Cytochrome P450 cDNA)
Synonym
Cytochrome P450, family 2, subfamily D, polypeptide 6
Substrate
Fluorescent substrate
Assay Properties
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (µM) |
|---|---|
| Ketoconazole | 1.76 |
| Proadifen (SKF-525A) | 0.03 |
| Aminobenzotriazole | 4.00 |
Scientific Information
NP_000097(http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=40805836)
Screening Location
Malvern, USA
Further Information
More information can be found on our website Cytochrome P450 Assay Services.
Reference compound IC50 for Cyp2D6
