CYP2D6 for compound screening and profiling via fluorescence-based cytochrome P450 inhibition assay quantification of enzyme activity.
Target Overview
CAT#
CYP2D6
Enzyme
Cyp2D6 + NADPH-P450 Reductase (microsomes from insect cells infected with baculovirus containing the human CYP2D6 cDNA and rabbit Cytochrome P450 cDNA)
Synonym
Cytochrome P450, family 2, subfamily D, polypeptide 6
Substrate
Fluorescence Substrate Vivid® EOMCC
Assay Properties
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (µM) |
|---|---|
| Ketoconazole | 6.08 |
| Proadifen (SKF525A) | 0.03 |
| Aminobenzotriazole | 4.00 |
Scientific Information
P10635 (https://www.uniprot.org/uniprotkb/P10635/entry)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2D6
