CYP2D6 for compound screening and profiling assay using luminescence-based method to measure cytochrome P450 enzyme activity.
Target Overview
CAT#
CYP2D6
Enzyme
Human CYP2D6 + P450 Reductase microsomes
Synonym
CYP2D6; cytochrome P450 family 2, subfamily D, polypeptide 6; debrisoquine 4-hydroxylase
Scientific Information
P10635 (https://www.uniprot.org/uniprotkb/P10635/entry)
Assay Properties
Substrate
P450-Glo™ proluciferin Substrate
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + oxidized [NADPH—hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2D6
