CYP2E1 for compound screening and profiling via fluorescence-based cytochrome P450 inhibition assay quantification of enzyme activity.
Target Overview
CAT#
CYP2E1
Enzyme
CYP2E1 + NADPH-P450 Reductase (microsomes from insect cells infected with baculovirus containing the human CYP2E1 cDNA and rabbit Cytochrome P450 cDNA)
Synonym
Cytochrome P450, family 2, subfamily E, polypeptide 1, CYP 2E1
Substrate
Fluorescence Substrate Vivid® EOMCC
Assay Properties
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (µM) |
|---|---|
| Ketoconazole | >100 |
| Tranylcypromine | 97.4 |
| Terfenadine | >100 |
Scientific Information
P05181 (https://www.uniprot.org/uniprotkb/P05181/entry)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2E1
