CYP2E1 for compound screening and profiling assay using luminescence-based method to measure cytochrome P450 enzyme activity.
Target Overview
CAT#
CYP2E1
Enzyme
Human CYP2E1 + P450 Reductase microsomes
Synonym
CYP2E1; cytochrome P450 family 2, subfamily E, polypeptide 1; 4-nitrophenol 2-hydroxylase
Scientific Information
P05181 (https://www.uniprot.org/uniprotkb/P05181/entry)
Assay Properties
Substrate
P450-Glo™ proluciferin Substrate
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + oxidized [NADPH—hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Nifedipine | 90010 |
| Tranylcypromine | 32300 |
| Ketoconazole | >10000 |
| Miconazole | >10000 |
| Verapamil | >10000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2E1
