CYP2J2 for compound screening and profiling assay using luminescence-based method to measure cytochrome P450 enzyme activity.
Target Overview
CAT#
CYP2J2
Enzyme
Human CYP2J2 + P450 Reductase microsomes
Synonym
CYP2J2; cytochrome P450, family 2, subfamily J, member 2; Arachidonic acid epoxygenase, Hydroperoxy icosatetraenoate isomerase
Scientific Information
P51589 (https://www.uniprot.org/uniprotkb/P51589/entry)
Assay Properties
Substrate
P450-Glo™ proluciferin Substrate
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + oxidized [NADPH—hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Ketoconazole | 23.5 |
| Miconazole | 2600 |
| Verapamil | >10000 |
| Tranylcypromine | >10000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2J2
