CYP2J2 for compound screening and profiling via fluorescence-based cytochrome P450 inhibition assay quantification of enzyme activity.
Target Overview
CAT#
CYP2J2
Enzyme
CYP2J2 + NADPH-P450 Reductase (microsomes from insect cells infected with baculovirus containing the human CYP2J2 cDNA and rabbit Cytochrome P450 cDNA)
Synonym
Cytochrome P450, family 2, subfamily J, member 2
Substrate
Fluorescence Substrate Vivid® MOBFC
Assay Properties
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (µM) |
|---|---|
| Ketoconazole | 0.43 |
| Tranylcypromine | 24 |
| Terfenadine | 5.9 |
Scientific Information
P51589 (https://www.uniprot.org/uniprotkb/P51589/entry)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP2J2
