CYP3A4 for compound screening and profiling assay using luminescence-based method to measure cytochrome P450 enzyme activity.
Target Overview
CAT#
CYP3A4
Enzyme
Human CYP3A4 + P450 Reductase microsomes
Synonym
CYP3A4, cytochrome P450 subfamily IIIA polypeptide 4
Scientific Information
P08684 (https://www.uniprot.org/uniprotkb/P08684/entry)
Assay Properties
Substrate
P450-Glo™ proluciferin Substrate
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + oxidized [NADPH—hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Ketoconazole | 4.6 |
| Verapamil | 6810 |
| Montelukast | 1730 |
| Tranylcypromine | >10000 |
| Cimetidine | >10000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP3A4
