CYP3A5 for compound screening and profiling assay using luminescence-based method to measure cytochrome P450 enzyme activity.
Target Overview
CAT#
CYP3A5
Enzyme
Human CYP3A5 + P450 Reductase microsomes
Synonym
CYP3A5; cytochrome P450, subfamily IIIA, polypeptide 5
Scientific Information
P20815 (https://www.uniprot.org/uniprotkb/P20815/entry)
Assay Properties
Substrate
P450-Glo™ proluciferin Substrate
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + oxidized [NADPH—hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Ketoconazole | 171 |
| Imipramine | >10000 |
| Verapamil | >10000 |
| Montelukast | >10000 |
| Tranylcypromine | >10000 |
| Cimetidine | >10000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP3A5
