DAPK1 Kinase Assay Service

Activity-based DAPK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
DAPK1

Enzyme
Human DAPK1

HGNC
DAPK1

Synonym
DAPK1

Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4.3
Ro 31-8220 1,400
H-89 8,700
Scientific Information
P53355 (http://www.uniprot.org/uniprot/P53355)

Screening Location
Malvern, USA

Reference compound IC50 for DAPK1
Reference compound IC50 for DAPK1

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