DAPK2 Kinase Assay Service

Activity-based DAPK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
DAPK2

Enzyme
Human DAPK2

HGNC
DAPK2

Synonym
DRP-1

Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 10.5
Ro 31-8220 6,900
GW 5074 4,400
Scientific Information
Q9UIK4 (http://www.uniprot.org/uniprot/Q9UIK4)

Screening Location
Malvern, USA

Reference compound IC50 for DAPK2
Reference compound IC50 for DAPK2

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