Activity-based DCAMKL1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DCAMKL1
Enzyme
Human DCAMKL1
HGNC
DCLK1
Synonym
DCLK, KIAA0369
Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 120 |
| Ro 31-8220 | 20,000 |
| GW5074 | >50,000 |
| H-89 | >50,000 |
| Rottlerin | >50,000 |
Scientific Information
O15075 (http://www.uniprot.org/uniprot/O15075)
Screening Location
Malvern, USA
Reference compound IC50 for DCAMKL1
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