DDR1 Kinase Assay Service

Activity-based DDR1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
DDR1

Enzyme
Human DDR1

HGNC
DDR1

Synonym
CAK, CD167, DDR, EDDR1, MCK10, NEP

Substrates
Peptide substrate, [KKSRGDYMTMQIG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.0
Ro 31-8220 5.7
GW 5074 5.1
H-89 18,000
Rottlerin 1,000
Scientific Information
Q08345 (http://www.uniprot.org/uniprot/Q08345)

Screening Location
Malvern, USA

Reference compound IC50 for DDR1
Reference compound IC50 for DDR1

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