DDR2 (T654M) Kinase Assay Service

Activity-based DDR2 (T654M) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DDR2 (T654M)
Enzyme
Human DDR2 (T654M)
HGNC
DDR2 (T654M)
Synonym
MIG20a, NTRKR3, TKT, TYRO10
Substrates
Peptide substrate, [KKSRGDYMTMQIG], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine0.6
PP260,000
AG1478>100,000
PDGFR I-III>100,000
D-6440648,000
Scientific Information
Q16832 (http://www.uniprot.org/uniprot/Q16832)
Screening Location
Malvern, USA
Reference compound IC50 for DDR2 (T654M)
Reference compound IC50 for DDR2 (T654M)