Activity-based DGKH lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
DGKH
Enzyme
Human DGKH
HGNC
DGKH
Synonym
Diacylglycerol kinase eta, DAG kinase eta, DGK-eta
Scientific Information
Q86XP1 (https://www.uniprot.org/uniprot/Q86XP1)
Assay Properties
Substrates
DLG (1,2-Dilauroyl-sn-glycerol), 500 µM
ATP, 50 µM
Reaction
1,2-dilauroyl-sn-glycerol + ATP = 1,2-dilauroyl-sn-glycero-3-phosphate + ADP + H+
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| DGK-IN-1 (CAS No: 2407892-34-4) | >100,000 |
| Calphostin C | 3,034 |
| R-59-949 | >100,000 |
| R-59-022 | 82,000 |
| Cochlioquinone A | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for DGKH
PDF