DGKK Kinase Assay Service

Activity-based DGKK lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.

Target Overview

CAT#

DGKK

Enzyme

Human DGKK

HGNC

DGKK

Synonym

Diacylglycerol kinase kappa, DAG kinase kappa, DGK-kappa, 142 kDa diacylglycerol kinase

Scientific Information

Q5KSL6 (https://www.uniprot.org/uniprot/Q5KSL6)

Assay Properties

Substrates

DLG (1,2-Dilauroyl-sn-glycerol), 500 µM + Phosphatidylserine, 0.1 mg/mL
ATP, 200 µM

Reaction

1,2-dilauroyl-sn-glycerol + ATP = 1,2-dilauroyl-sn-glycero-3-phosphate + ADP + H+

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
DGK-IN-1 (CAS No: 2407892-34-4) 3,100
Calphostin C 30,100

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for DGKK

Reference compound IC50 for DGKK