Activity-based DGKQ lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
DGKQ
Enzyme
Human DGKQ
HGNC
DGKQ
Synonym
Diacylglycerol kinase theta, DAG kinase theta, DGK-theta, DAGK4
Scientific Information
P52824 (https://www.uniprot.org/uniprot/P52824)
Assay Properties
Substrates
DLG (1,2-Dilauroyl-sn-glycerol), 500 µM
ATP, 50 µM
Reaction
1,2-dilauroyl-sn-glycerol + ATP = 1,2-dilauroyl-sn-glycero-3-phosphate + ADP + H+
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| DGK-IN-1 (CAS No: 2407892-34-4) | >100,000 |
| Calphostin C | 3,700 |
| R-59-949 | >100,000 |
| R-59-022 | >100,000 |
| Cochlioquinone A | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for DGKQ
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