DGKZ Kinase Assay Service

Activity-based DGKZ lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.

Target Overview

CAT#

DGKZ

Enzyme

Human DGKZ

HGNC

DGKZ

Synonym

Diacylglycerol kinase zeta, DAG kinase zeta, DGK-zeta, DAGK6

Scientific Information

Q13574 (https://www.uniprot.org/uniprot/Q13574)

Assay Properties

Substrates

DLG (1,2-Dilauroyl-sn-glycerol), 500 µM
ATP, 50 µM

Reaction

1,2-dilauroyl-sn-glycerol + ATP = 1,2-dilauroyl-sn-glycero-3-phosphate + ADP + H+

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
DGK-IN-1(CAS No: 2407892-34-4) 22.9
Calphostin C 10,300
R-59-949 >100,000
R-59-022 >100,000
Cochlioquinone A >100,000

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for DGKZ

Reference compound IC50 for DGKZ