DLK/MAP3K12 Kinase Assay Service

Activity-based DLK/MAP3K12 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DLK/MAP3K12
Enzyme
Human DLK/MAP3K12
HGNC
MAP3K12
Synonym
DLK
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine21
Ro 31-822024,000
GW 507421,000
H-8935,000
Rottlerin>100,000
Scientific Information
Q12852 (http://www.uniprot.org/uniprot/Q12852)
Screening Location
Malvern, USA
Reference compound IC50 for DLK/MAP3K12
Reference compound IC50 for DLK/MAP3K12