Activity-based DMPK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DMPK
Enzyme
Human DMPK
HGNC
DMPK
Synonym
DMPK1, DM, DM1, DM1PK, DMK
Substrates
Peptide substrate, [KKRNRRLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 22.7 |
| Ro 31-8220 | 4,000 |
| GW 5074 | >100,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q09013 (http://www.uniprot.org/uniprot/Q09013)
Screening Location
Malvern, USA
Reference compound IC50 for DMPK
PDF