DNA-PK Kinase Assay Service

Activity-based DNA-PK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
DNA-PK

Enzyme
Human DNA-PK

HGNC
PRKDC

Synonym
DNPK1, HYRC, p350

Substrates
Peptide substrate, [EPPLSQEAFADLWKK], 20 μM
DNA, 10 μg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
LY294002 450
Wortmannin 43
PI3K I 56
PI-103 12
Scientific Information
P78527 (http://www.uniprot.org/uniprot/P78527)

Screening Location
Malvern, USA

Reference compound IC50 for DNA-PK
Reference compound IC50 for DNA-PK

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