DNMT1 Methyltransferase Assay Service

Activity-based DNMT1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
DNMT1
Enzyme
Human DNMT1
Synonym
DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, MCMT, AIM, CXXC9, DNMT
Substrates
Poly(dI-dC), 0.001 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + DNA = S-adenosyl-L-homocysteine + DNA 5-[methyl-³H]-cytosine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH270
Sinefungin7,400
Scientific Information
P26358 (http://www.uniprot.org/uniprot/P26358)
Screening Location
Malvern, USA
Reference compound IC50 for DNMT1
Reference compound IC50 for DNMT1