DNMT3A Methyltransferase Assay Service

Activity-based DNMT3A Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
DNMT3A
Enzyme
Human DNMT3A
Synonym
DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA, DNM3A
Substrates
Lambda DNA, 0.0075 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + DNA = S-adenosyl-L-homocysteine + DNA 5-[methyl-³H]-cytosine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH271
Sinefungin430
Scientific Information
Q9Y6K1 (http://www.uniprot.org/uniprot/Q9Y6K1)
Screening Location
Malvern, USA
Reference compound IC50 for DNMT3A
Reference compound IC50 for DNMT3A